A. absorbance of analyte solution is kept near to 0.05
B. oxygen is eradicated from solution
C. pH of solution is controlled
D. wavelength of incident light is always above 400 nm
Correct from the above is
(a)A, B and D
(b)B, C and D
(c)A, B and C
(d)A, C and D
Answer
Answer (as printed): C
Explanation
(a) In spectrofluorimetry the wavelength of incident light can range from 200 nm to 900 nm.(b) Intensity and colour of the fluorescence of many substance depends upon the pH of solutions.(c) Since, presence of $\mathrm{O_2}$ decreases the fluorescence, thus oxygen is eradicated from the solution.(d) In flourimetry absorbance of analyte solution is generally kept near to 0.05.