Which of the following will result in deviation from Beer's law ? 1. Change in refractive index or medium. 2. Dissociation of analyte on dilution. 3. Polychromatic light. 4. Path length of cuvette.
(a)1, 2 and 3
(b)2, 3 and 4
(c)1,3 and 4
(d)1, 2 and 4
Answer
Answer: A ✓ checked by 4AB · confidence high
The source book printed no answer; this one was worked out and checked — see the explanation.
Explanation
Real deviations from Beer's law come from changes in refractive index at high concentration, chemical dissociation or association, and polychromatic radiation. Path length is part of the law itself. 1, 2 and 3 (a).