A. absorbance of analyte solution is kept near to 0.05
B. oxygen is eradicated from solution
C. pH of solution is controlled
D. wavelength of incident light is always above 400 nm
Correct from the above is
(a)A, B and D
(b)B, C and D
(c)A, B and C
(d)A, C and D
Answer
Answer: C ✓ checked by 4AB · confidence high
Answer: A, B and C — code (3).
Explanation
Answer: A, B and C — code (3) — Absorbance is kept low (≈0.05) to avoid inner-filter effects; dissolved O₂ (a quencher) is removed; pH is controlled because the emitting species is often pH-dependent. The excitation wavelength is chosen at the analyte's absorption band and need not be above 400 nm.