Inspectrofluorimetric determination in solutionA. absorbance of analyte solution is kept near to 0.05B. oxygen is eradicated from solutionC. pH of solution is controlledD. wavelength of incident light is always above 400 nm
(a)A, B and D
(b)B, C and D
(c)A, B and C
(d)A, C and D
Answer
Answer: C ✓ checked by 4AB · confidence high
Explanation
For spectrofluorimetry, absorbance is kept low (~0.05) to avoid inner-filter effects, quenching O₂ is removed, and pH is controlled. The excitation λ is chosen at the absorption band, not necessarily above 400 nm. A, B and C (c).