Identify the correct statement(s) for phosphorimetric measurement from the following:A. It is done after a time delay when fluorescence, if present becomes negligibleB. Immobilization of analyte increases phosphorescence.C. Phosphorescence decreases in the presence of heavy atoms
(a)A only
(b)A and B
(c)A and C
(d)B and C
Answer
Answer: B ✓ checked by 4AB · confidence high
Explanation
Phosphorescence is read after a delay when fluorescence has died (A true); immobilising the analyte (frozen matrix, adsorption) suppresses non-radiative decay and raises it (B true); heavy atoms increase intersystem crossing and enhance, not decrease, phosphorescence (C false). A and B.