Q39 · CSIR NET Chemistry, December 2015
Paper: CSIR NET December 2015 · Subject: Inorganic Chemistry · Chapter: Analytical Chemistry · Topic: Sampling Calibration · Marks: 2 · Difficulty: Easy
The calibration curve in spectrofluorimetric analysis becomes non-linear when
(a)molecular weight of analyte is high
(b)intensity of light source is high
(c)concentration of analyte is high
(d)molar absorptivity of analyte is high
Answer
Answer: C ✓ checked by 4AB · confidence high
The source book printed A; on checking, C is correct — see the explanation.
Book printed (a).
Explanation
At high analyte concentration inner-filter effects and self-absorption make fluorescence non-linear (c).
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