An aqueous solution of hemoglobin has a molar absorptivity value of 18,600 L mol⁻¹ cm⁻¹ for an absorbance value of 0.1 at 540 nm (Given: cell thickness = 1 cm). The concentration (in) of the haemoglobin solution is

(b) 5.37. Explanation: c = A/εl = 0.1/18600 = 5.37×10⁻⁶ M = 5.37 µM (b).

An aqueous solution of hemoglobin has a molar absorptivity value of 18,600 L mol⁻¹ cm⁻¹ for an absorbance… · ABC26PH0668

Subject: Physical Chemistry · Chapter: Chemical Kinetics · Topic: Chemical Kinetics – General · Exam: JAM 2001-2022 · Marks: 2 · Difficulty: Medium

An aqueous solution of hemoglobin has a molar absorptivity value of $18{,}600\,\mathrm{L\,mol^{-1}\,cm^{-1}}$ for an absorbance value of 0.1 at 540 nm (Given: cell thickness $= 1\,\mathrm{cm}$). The concentration (in $\mu\mathrm{M}$) of the haemoglobin solution is

(a)0.537

(b)5.37

(c)53.7

(d)537.0

Answer

Answer: B ✓ checked by 4AB · confidence high

Explanation

c = A/εl = 0.1/18600 = 5.37×10⁻⁶ M = 5.37 µM (b).

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